RNA Preparation and Purification
Biochain Institute Inc Total RNA - hypertension: Heart Atrium, right, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Liver Cirrhosis: Spleen, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Diabetic Disease: Stomach, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Epoch Life Science Inc GenCatch™ Plant RNA Purification System (50 preps) Uniquely formulated buffers, shearing tubes, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify total RNA from plant (and fungal) samples. Comes with shearing tubes for simple and fast homogenization and filtration of tissue. Total RNA with a molecular size greater than 200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can be isolated. Silica membrane technology. Up to 100 ug yield. 50 ul elution volume. 100 mg sample size. Additional buffer provided for handling plant tissue that contains sticky secondary metabolites. RNA isolated can be used for a variety of molecular manipulations such as Northern blotting, RT-PCR, PolyA+ RNA selection, cDNA synthesis, Primer extension, in vitro translation, etc. No phenol/chloroform extraction nor ethanol precipitation required. Used by labs around the world. 18 month shelf life.
Norgen Biotek Corp RNA/Protein Purification Plus Kit, Spin column method, 30-35 μg RNA yield (10 mg tissue), 100-150 μg protein yield (10 mg tissue), 50 preps
The RNA/Protein Purification Plus Kit provides a rapid and efficient spin column-based method for the sequential isolation and purification of total RNA and proteins from biological samples without hazardous reagents. This kit enables researchers to conduct analyses of both the transcriptome and proteome from difficult-to-obtain samples, ensuring high-quality recovery of macromolecules.
- Rapid protocol with hands-on time under 30 minutes
- Efficient isolation of total RNA, including all sizes like mRNA and miRNA
- Compatible with various sample types: animal cells, tissues, blood, bacteria, yeast, fungi, and plants
- Includes dedicated gDNA removal technology for DNA-free RNA
- Yield of 30-35 μg RNA and 100-150 μg protein from 10 mg hamster liver
- Suitable for diverse downstream applications in RNA and protein analysis
- Shelf life of 1 year after shipment
- 50 preps included in the kit
Agilent Technologies High Sensitivity RNA Kit, 1000 samples, 200 to 6000 nt sizing range, 2 μL sample volume
The DNF-472-1000 High Sensitivity RNA Kit is designed for advanced analysis of total RNA and IVT mRNA with Agilent Fragment Analyzer systems. It provides precise RNA quality assessment, crucial for applications such as vaccine development, qPCR, and NGS. This kit allows for reliable sizing and quantification of RNA samples, ensuring its usefulness in various research applications.
- High sensitivity analysis optimized for low-concentration RNA samples.
- Accurate sizing from 200 to 6,000 nt with precise quantification.
- Objective quality assessment using RNA Quality Number (RQN).
- Compatible with Agilent Fragment Analyzer systems for efficient workflow.
- Requires only 2 μL of sample for testing.
Zymo Research Corporation Quick-RNA™ MiniPrep Kit (50 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The procedure of Quick-RNA™ Miniprep Kit uses unique spin-column technology that results in highquality total RNA (including small/microRNAs) and is ready for Next-Gen Sequencing, RT/qPCR, hybridization, etcThe Quick-RNA™ Kits yields high quality total RNA.High levels of genomic DNA contamination arepresent in the preps from Suppliers Q & P but not withthe Quick-RNA™ Kits. Total RNA was isolated fromhuman epithelial cells.RNA isolated with the Quick-RNA™ Kits is DNA-free.Samples isolated with Supplier Q's kit are provided forcomparison. Total RNA was isolated from 106 humanepithelial cells (with in-column DNase treatments forboth kits). Each amplification curve represents anaverage of three independent isolation experiments.
Zymo Research Corporation Quick-RNA™ MicroPrep Kit (200 Preps) w/Zymo-Spin™ IC Columns (Capped)
The Quick-RNA Microprep Kit is designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin™Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Zymo Research Corporation Quick-RNA™ MiniPrep Kit (200 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The procedure of Quick-RNA™ Miniprep Kit uses unique spin-column technology that results in highquality total RNA (including small/microRNAs) and is ready for Next-Gen Sequencing, RT/qPCR, hybridization, etcThe Quick-RNA™ Kits yields high quality total RNA.High levels of genomic DNA contamination arepresent in the preps from Suppliers Q & P but not withthe Quick-RNA™ Kits. Total RNA was isolated fromhuman epithelial cells.RNA isolated with the Quick-RNA™ Kits is DNA-free.Samples isolated with Supplier Q's kit are provided forcomparison. Total RNA was isolated from 106 humanepithelial cells (with in-column DNase treatments forboth kits). Each amplification curve represents anaverage of three independent isolation experiments.
New England Biolabs, Inc. Ribonucleotide Solution Mix – 50 µmol of each
Ribonucleotide Solution Mix is a buffered equimolar solution of ribonucleotide triphosphates: rATP, rCTP, rGTP and rUTP, pH 7.5 as sodium salts. The NTPs are also available as Ribonucleotide Solution Set . Supplied as: Sodium salts, pH 7.5 Reagents Supplied (small): NTP Mix (25 mM of each) 0.4 ml Reagents Supplied (large): NTP Mix (25 mM of each) 5 x 0.4 ml Diluent Compatibility: Can be diluted using nuclease-free water
Norgen Biotek Corp Urne Cellfree Circ Rna Minikit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples from volumes ranging from 250 µL to 2 mL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Zymo Research Corporation Quick-RNA Fungal/Bacterial MicroPrep™ Kit (50 Preps)
Ultra-high density ZR BashingBeads™ are included for sample homogenization coupled with a robust buffer system, delivering total RNA (including small/microRNAs). The Zymo-Spin™ IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IC Column efficiently binds total RNA.RNA is eluted in as little as 6 l and is suitable for subsequent procedures including RT-qPCR.